Cell Analysis, Sorting & Monoclonal Plating Device
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Cell Analysis, Sorting & Monoclonal Plating Device
WOLF cell analysis, sorting & monoclonal plating device is an all-in-one solution for cell analysis, cell sorting and monoclonal plating offered by NanoCellect Biomedical Inc. WOLF's gentle sorting mechanism (< 2 psi), low shear stress and large particles (900nm-65μm) sorting allow for more effective sorting of rare target cells with high activity. During the sorting process, cell culture medium, PBS and even seawater can be used as sheath fluid to ensure cell viability while enhancing monoclonal sorting efficiency.
Nondestructive sorting of fragile cells, such as primary cells, protoplasts and nucleus
• Purity of enriched and sorted cells >99%, cell viability >95%, cell viability 24 hours after sorting >90%, cell viability one week after sorting >85%；single-cell plating efficiency >95%, monoclonal outgrowth efficiency >90%
Can be used in biosafety level-3 labsP3
• With a size of a laptop, it can easily be placed into a biosafety cabinet to avoid contamination • Microfluidic sorting cartridges help avoid the risk of cross-contamination • <2psi low-pressure sorting to prevent formation of aerosols • Cartridges (>100 96-well plates for single cartridge) can automatically clean and change samples
Intelligent control throughout the process, no maintenance
• Integrated software for flow analysis + flow sorting, with no limitation on number of accounts. “Next-step” operation can be learned within an hour • Universal FCS3.1 format can generate scatter plots, bar plots, contour plots, heat maps and overlay plots, etc. • Index Sorting module for “one-button” tracking of monoclonal results • 100,000-hour warranty for laser with fixed optical path design, no consumable part and can be freely moved among labs • All fluid paths are embedded in cartridge, thus requiring no cleaning before shut down, and there is no need for fluid container
Application scenario
Single-cell sequencing
Cell line development
Antibody development
Gene editing
Monoclonal outgrowth of T-cell
Monoclonal outgrowth of B-cell
Monoclonal outgrowth of iPS
Sorting of sensitive and fragile cells
Sorting of 65μm particles
Sorting of infectious samples
Technical Specifications
| Product name | Cell Analysis, Sorting & Monoclonal Plating Device |
| Sorting pressure | Less than 0.138bar (1/7 bar, gentle sorting) |
| Principle of sorting | Microfluidic cartridges-based sorting |
| Sample input | 1.5mL tube, 5mL tube and 50mL conical tube |
| Sorting output | 1.5mL/5mL, 96/384-well plates, 96/384-well PCR plates |
| Dead volume | 50μL |
| Minimum sample volume | 150μL |
| Sample flow rate | 24μL/min |
| Sheath flow rate | 160μL/min |
| Sheath fluid usage | 7.5mL/hour |
| Fluid path flow rate | 9.6mL/hour |
| Sorting system | Sorting of stock cultures of cells, growth medium recommended, buffer not required |
| Cell count | Absolute count, accurate to single cell |
| Cell sorting | Cell purity > 99%, cell viability > 95% |
| Single-cell plating time | 2-8 min (96-well plate); 9-30 min (384-well plate) |
| Volume of single-cell droplet | 3.5-8μL |
| G2 dimensions and weight | Side length not exceeding 40cm, 32kg |
| WOLF dimensions and weight | Side length not exceeding 40cm, 20kg |
| N1 dimensions and weight | 21cm×16cm×20cm, 3kg |
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